QuantiChrom™ Biotin Assay Kit

Application

• Quantitative determination of biotin in food, cosmetic, and biotinylated proteins.

Key Features

• Fast and sensitive. Linear detection range: 8 to 200 µM biotin with 30 µL sample (96-well) or 10 to 200 µM biotin with 10 µL sample (384-well).
• Convenient. The procedure involves adding a single working reagent and reading after 20 minutes.
• High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day.

Method

•  OD500nm

Samples

•  Food, cosmetics, supplements, and biotinylated proteins or antibodies

Species

•  All

Size

•  100 tests (96-well), 320 tests (384-well)

Detection Limit

•  8 μM

Shelf Life

•  12 months

More Details

•  BIOTIN , or Vitamin B7, is a water soluble vitamin involved in metabolism, cell growth, and protein synthesis. It is a cofactor to multiple carboxylases necessary for metabolizing fatty acids, glucose, and amino acids. Biotin is found in a wide range of foods and is often taken as a dietary supplement. In the biotechnology industry, biotin is commonly conjugated to proteins in a process called biotinylation. These biotinylated proteins can then be specifically selected using streptavidin and/or avidin's strong affinity for biotin in biochemical assays such as ELISAs.
  
  BioAssay Systems' Biotin Assay Kit is based on avidin's weak affinity for 4'-hydroxyazobenzene-2-carboxylic acid (HABA) and strong affinity for biotin. The avidin-HABA complex reagent has an absorbance at 500nm. When the colored avidin-HABA reagent is introduced to biotin, the biotin binds to avidin, displacing the HABA and causing a decrease in absorbance. The decrease in absorbance at 500nm is directly proportional to the biotin in the sample.