Bioassay Systems
QuantiChrom™ Carbonyl Assay Kit
- SKU:
- DCAR-100
- Availability:
- In Stock
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
QuantiChrom™ Carbonyl Assay Kit | DCAR-100 | Bioassay Systems
Quantification of carbonyl groups or protein carbonyls in biological samples.
• Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 min.
• High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
CARBONYL groups, such as ketones and aldehydes, are a commonindicator of protein oxidation. Protein oxidation is caused as a result of exposure to reactive oxygen species (ROS) and is a common marker in various diseases as well as aging. BioAssay Systems' Carbonyl Assay Kit is based on an improved method, where 2,4-dinitrophenylhydrazine (DNPH) reacts with carbonyl groups to produce a colored compound at 375 nm. The intensity of this colored compound is directly proportional to the carbonyl groups in the sample.