QuantiChrom™ Hydroxyproline Assay Kit

• Safe. Our hydroxyproline assay uses an improved perchlorate-free chemistry.
• Sensitive and accurate. Uses 20 µL sample. Linear detection range 0.5 µg/mL to 50 µg/mL hydroxyproline in 96-well plate assay.
• Convenient. Collagen can be hydrolyzed in Eppendorf tubes, and final incubation can be performed at 37°C.

HYDROXYPROLINE is a unique modified amino acid that is found exclusively in several animal proteins, the most prevalent of which is collagen. Collagen is a key structural protein of the extracellular matrix and is common in connective tissues due to its incredible strength. Because of its role as a key structural protein in animals collagen is crucial for many medical applications, such as tissue transplantation and scaffolding for complex cell cultures. Collagen is also used in the beauty industry, as it can be supplemented to increase tissue rigidity, resulting in skin that appears more youthful. BioAssay Systems’ Hydroxyproline assay kit delivers a safe, simple, and sensitive means to quantify hydroxyproline in samples. In the first step of this procedure, hydroxyproline in the sample is oxidized to a pyrrole ring. This compound then reacts with a dye reagent to yield a pink product that can be measured at 560 nm. Hydroxyproline exists almost exclusively in collagen, so hydroxyproline content can be used as a proxy for collagen content.