TIM3 Antibody [2A6]

TIM3 Antibody [2A6] | RF16106 | ProSci

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: TIM-3 antibody was raised against the extracellular domain of human TIM-3

Research Area: Cell Cycle, Cancer, Immunology

Tested Application: E, WB, IHC-P, ICC, IF, Flow

Application: TIM-3 antibody can be used for ELISA starting at 0.1 μg/mL. For Western blot start at 1 μg/mL. For Immunocytochemistry start at 1 μg/mL. For Immunofluorescence start at 10 μg/mL. For Immunohistochemistry start at 5 μg/mL. For Flow Cytometry start at 0.1 μg/ml.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 33 kDa
Observed: 37 kDa

Validation: N/A

Isoform: N/A

Purification: TIM-3 Antibody is supplied as protein A purified IgG1.

Clonality: Monoclonal

Clone: 2A6

Isotype: IgG1, k

Conjugate: Unconjugated

Physical State: Liquid

Buffer: TIM-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/mL

Storage Condition: TIM-3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TIM-3 Antibody: Hepatitis A virus cellular receptor, HAVCR2, TIM3, CD366, KIM-3, TIMD3, TIMD-3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The immune checkpoint protein TIM3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1) . TIM3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2, 3) .