Girijesh K Patel
Size distribution and Polydispersity index (PDI) analysis of using dynamic light scattering. To determine the size distribution, freshly isolated exosomes were subjected to dynamic light scattering measurements using DelsaMax Pro (Backman Coulter Inc.). Exosomes isolated using Ultracentrifugation, 101Bio (PureExo), MagCapture and iZON gel-filtration, were under the size range (
Exosomes have received significant attention for their role in pathobiological processes and are being explored as a tool for disease diagnosis and management. Consequently, various isolation methods based on different principles have been developed for exosome isolation. Here we compared the efficacy of four kits from Invitrogen.
size distribution, freshly isolated exosomes from different methods were subjected to dynamic light scattering measurements, DelsaMax Pro (Beckman Coulter, Indianapolis, IN, USA). All the exosome preparations from different isolation methods showed the accepted size range (