Biochemical characterization of exosome preparations: exosome preparations were obtained from 1 mL MM pool with four different protocols. Samples were electrophoresed and analyzed by Western Blot (WB) for the presence of typical vesicular markers. (A) Exosome preparation obtained with differential centrifugation steps (P3). (B) Exosome preparation purified with a precipitation kit (Exo PK). (C) Exosome preparation obtained with discontinuous iodixanol gradient. Top numbers refer to the corresponding gradient fraction. (D) Exosome preparation obtained with discontinuous sucrose gradient. Top numbers refer to the corresponding gradient fraction.
Exosomes are gaining a prominent role in research due to their intriguing biology and several therapeutic opportunities. However, their accurate purification from body fluids and detailed physicochemical characterization remain open issues. We isolated exosomes from serum of patients with Multiple Myeloma by four of the most popular purification me.