rneasy mini kit
RNeasy Mini Kit
What is the RNeasy Mini Kit?
The RNeasy Mini Kit is a silica-membrane RNA purification system designed for rapid isolation of high-quality total RNA from cells, tissues, and microorganisms. Widely used in molecular biology, transcriptomics, and clinical research laboratories, it ensures reproducible and RNase-free RNA suitable for downstream applications.
How It Works (Step-by-Step)
- Cell Lysis: Sample is lysed with a guanidine-thiocyanate buffer to inactivate RNases and preserve RNA.
- RNA Binding: Ethanol is added, RNA binds selectively to the silica membrane.
- Washing: Impurities like proteins, DNA, and lipids are removed.
- Elution: Pure RNA is eluted in RNase-free water.
Applications
- RT-qPCR
- RNA-Seq / Transcriptomics
- Microarray analysis
- Northern blotting
- cDNA synthesis & Gene expression profiling
Technical Specifications
| Feature | Specification |
|---|---|
| Sample Type | Cells & Tissues |
| Maximum Input | 1 × 10⁷ cells |
| RNA Size | ≥ 200 nucleotides |
| Yield | Up to 100 µg total RNA |
| Purity | A260/280 ~2.0 |
| Processing Time | ~30 minutes |
| RNase-Free | Yes |
Comparison vs Other RNA Kits
| Feature | RNeasy Mini Kit | TRIzol | Magnetic Beads |
|---|---|---|---|
| Hands-on time | Low | High | Medium |
| RNA Purity | Very High | Variable | High |
| Phenol-free | ✅ Yes | ❌ No | ✅ Yes |
| Automation | Limited | ❌ No | ✅ Yes |
| Safety | High | Low | High |
Why Buy the RNeasy Mini Kit?
The RNeasy Mini Kit is perfect for laboratories requiring high-quality RNA for transcriptomics, qPCR, and RNA-Seq. Trusted by scientists worldwide, it offers reproducibility, safety, and speed.
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