Ang1-7(Angiotensin 1-7) Introduction

Angiotensin (1-7)  is an active heptapeptide of the renin–angiotensin system (RAS).Angiotensin (1-7) is a vasodilator agent that plays important roles in cardiovascular organs, such as heart, blood vessels, and kidneys having functions frequently opposed to those attributed to the major effector component of the RAS, angiotensin II (Ang II).

Human Ang1-7(Angiotensin 1-7) ELISA Kit Test method

This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

Reference:

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2. Schiavone MT, Santos RA, Brosnihan KB, Khosla MC, Ferrario CM (1988). "Release of vasopressin from the rat hypothalamo-neurohypophysial system by angiotensin-(1-7) heptapeptide". PNAS. 85 (11): 4095–8. Bibcode:1988PNAS...85.4095S. doi:10.1073/pnas.85.11.4095. PMC 280369. PMID 3375255.

3.Santos RA, Sampaio WO, Alzamora AC, Motta-Santos D, Alenina N, Bader M, et al. (2018). "The ACE2/Angiotensin-(1-7)/MAS Axis of the Renin-Angiotensin System: Focus on Angiotensin-(1-7)". Physiol Rev. 1 (98): 505–553. doi:10.1152/physrev.00023.2016. PMC 7203574. PMID 29351514.

4.Chappell MC (January 2016). "Biochemical evaluation of the renin-angiotensin system: the good, bad, and absolute?". American Journal of Physiology. Heart and Circulatory Physiology. 310 (2): H137-52. doi:10.1152/ajpheart.00618.2015. PMC 4796631. PMID 26475588.

5.Wilson BA, Nautiyal M, Gwathmey TM, Rose JC, Chappell MC (April 2016). "Evidence for a mitochondrial angiotensin-(1-7) system in the kidney". American Journal of Physiology. Renal Physiology. 310 (7): F637–F645. doi:10.1152/ajprenal.00479.2015. PMC 4824145. PMID 26697984.