ANP(Atrial Natriuretic Peptide) Introduction

Atrial natriuretic peptide (ANP) or atrial natriuretic factor (ANF) is a natriuretic peptide hormone secreted from the cardiac atria that in humans is encoded by the NPPA gene.Natriuretic peptides (ANP, BNP, and CNP) are a family of hormone/paracrine factors that are structurally related. The main function of ANP is causing a reduction in expanded extracellular fluid (ECF) volume by increasing renal sodium excretion. ANP is synthesized and secreted by cardiac muscle cells in the walls of the atria in the heart. These cells contain volume receptors which respond to increased stretching of the atrial wall due to increased atrial blood volume.

Human ANP(Atrial Natriuretic Peptide) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of ANP(Atrial Natriuretic Peptide).ANP(Atrial Natriuretic Peptide)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional ANP(Atrial Natriuretic Peptide) antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of ANP(Atrial Natriuretic Peptide) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

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