BNP(Brain Natriuretic Peptide) Introduction

Ventricular natriuretic peptide or brain natriuretic peptide (BNP), also known as B-type natriuretic peptide, is a hormone secreted by cardiomyocytes in the heart ventricles in response to stretching caused by increased ventricular blood volume. The 32-amino acid polypeptide BNP is secreted attached to a 76–amino acid N-terminal fragment in the prohormone called NT-proBNP (BNPT), which is biologically inactive. Once released, BNP binds to and activates the atrial natriuretic factor receptor NPRA, and to a lesser extent NPRB, in a fashion similar to atrial natriuretic peptide (ANP) but with 10-fold lower affinity. The biological half-life of BNP, however, is twice as long as that of ANP, and that of NT-proBNP is even longer, making these peptides better targets than ANP for diagnostic blood testing.

Human BNP(Brain Natriuretic Peptide) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of BNP(Brain Natriuretic Peptide. BNP(Brain Natriuretic Peptide-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional BNP(Brain Natriuretic Peptide antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of BNP(Brain Natriuretic Peptide in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference

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