Feiyuebio
Human IL-12(Interleukin 12) ELISA Kit
- SKU:
- FY-EH3935
- Weight:
- 0 KGS
- Shipping:
- Calculated at Checkout
Description
Human IL-12(Interleukin 12) Introduction
IL-12 is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils. IL-12 belongs to the family of interleukin-12. IL-12 family is unique in comprising the only heterodimeric cytokines, which includes IL-12, IL-23, IL-27 and IL-35]. Despite sharing many structural features and molecular partners, they mediate surprisingly diverse functional effects. IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.
Human IL-12(Interleukin 12) ELISA Kit Test Method
This kit uses sandwich ELISA to detect the concentration of Human IL-12.
Human IL-12-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human IL-12 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human IL-12 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.
Reference:
- Kaliński P, Hilkens CM, Snijders A, Snijdewint FG, Kapsenberg ML (July 1997). "IL-12-deficient dendritic cells, generated in the presence of prostaglandin E2, promote type 2 cytokine production in maturing human naive T helper cells". Journal of Immunology. 159 (1): 28–35. PMID 9200435.
- Vignali DA, Kuchroo VK (July 2012). "IL-12 family cytokines: immunological playmakers". Nature Immunology. 13 (8): 722–8. doi:10.1038/ni.2366. PMC 4158817. PMID 22814351.
- Hsieh CS, Macatonia SE, Tripp CS, Wolf SF, O'Garra A, Murphy KM (April 1993). "Development of TH1 CD4+ T cells through IL-12 produced by Listeria-induced macrophages". Science. 260 (5107): 547–9. doi:10.1126/science.8097338. PMID 8097338.
Additional Information
Size: |
96T |
Alias: |
Interleukin 12 p70 ELISA Kit, CLMF p35 ELISA Kit, Cytotoxic lymphocyte matuRation factor 35 kDa subunit ELISA Kit, IL-12 subunit p35 ELISA Kit, IL-12 ELISA Kit, subunit p35 ELISA Kit, interleukin 12 ELISA Kit, p35 ELISA Kit |
Application: |
IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 concentrations in serum, plasma, tissue homogenates and other biological fluids |
Storage: |
2-8 ℃ for 6 months |
Sensitivity: |
<9.375pg/ml |
Species: |
Human |
CV(%): |
Intra-Assay: CV<8% Inter-Assay: CV<10% |