Rat Hepc(Hepcidin) Introduction

Hepc is a protein that in Rats is encoded by the HAMP gene. Hepcidin is a key regulator of the entry of iron into the circulation in mammals. During conditions in which the hepcidin level is abnormally high, such as inflammation, serum iron falls due to iron trapping within macrophages and liver cells and decreased gut iron absorption. This typically leads to anemia due to an inadequate amount of serum iron being available for developing red blood cells. When the hepcidin level is abnormally low such as in hemochromatosis, iron overload occurs due to increased ferroportin mediated iron efflux from storage and increased gut iron absorption.Hepcidin exists as a preprohormone (84 amino acids), prohormone (60 amino acids), and hormone (25 amino acids). 

Rat Hepc(Hepcidin) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Rat Hepc.

Rat Hepc-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Rat Hepc antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming opticalsignal measured by microplate reader.The concentration of Rat Hepc in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

1. GRCh38: Ensembl release 89: ENSG00000105697 - Ensembl, May 2017.
2. "Rat PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
3. PDB: 1M4F​; Hunter HN, Fulton DB, Ganz T, Vogel HJ (October 2002). "The solution structure of Rat hepcidin, a peptide hormone with antimicrobial activity that is involved in iron uptake and hereditary hemochromatosis". J. Biol. Chem. 277 (40): 37597–603. doi:10.1074/jbc.M205305200. PMID 12138110.