Rat IFN-β(Interferon Beta) Introduction

IFN-β proteins are produced in large quantities by fibroblasts. They have antiviral activity that is involved mainly in innate immune response. Two types of IFN-β have been described, IFN-β1 (IFNB1) and IFN-β3 (IFNB3) (a gene designated IFN-β2 is actually IL-6). IFN-β1 is used as a treatment for multiple sclerosis as it reduces the relapse rate. The type-I interferons (IFN) are cytokines which play essential roles in inflammation, immunoregulation, tumor cells recognition, and T-cell responses. In the human genome, a cluster of thirteen functional IFN genes is located at the 9p21.3 cytoband over approximately 400 kb including coding genes for IFNα (IFNA1, IFNA2, IFNA4, IFNA5, IFNA6, IFNA7, IFNA8, IFNA10, IFNA13, IFNA14, IFNA16, IFNA17 and IFNA21), IFNω (IFNW1), IFNɛ (IFNE), IFNк (IFNK) and IFNβ (IFNB1), plus 11 IFN pseudogenes.

Rat IFN-β(Interferon Beta) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Rat IFN-β.

Rat IFN-β -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti- Rat IFN-β antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming opticalsignal measured by microplate reader.The concentration of Rat IFN-β in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

1. Razaghi, Ali; Brusselaers, Nele; Björnstedt, Mikael; Durand-Dubief, Mickael (2021-10-01). "Copy number alteration of the interferon gene cluster in cancer: Individual patient data meta-analysis prospects to personalized immunotherapy". Neoplasia. 23 (10): 1059–1068. doi:10.1016/j.neo.2021.08.004. ISSN 1476-5586. PMC 8458777. PMID 34555656.
2. Schultz U, Kaspers B, Staeheli P (May 2004). "The interferon system of non-mammalian vertebrates". Developmental and Comparative Immunology. 28 (5): 499–508. doi:10.1016/j.dci.2003.09.009. PMID 15062646.
3. Samarajiwa SA, Wilson W, Hertzog PJ (2006). "Type I interferons: genetics and structure". In Meager A (ed.). The interferons: characterization and application. Weinheim: Wiley-VCH. pp. 3–34. ISBN 978-3-527-31180-4.