LAG3 Antibody | 8655

LAG3 Antibody | 8655 | ProSci

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (65%)

Immunogen: LAG3 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human LAG3.
The immunogen is located within amino acids 110 - 160 of LAG3.

Research Area: Cell Cycle, Cancer, Immunology

Tested Application: E, WB, IHC-P, IF

Application: LAG3 antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least two antibodies are known to exist; this antibody will only detect the largest isoform.

Positive Control 1: Cat. No. 1404 - Mouse Liver Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 55 kDa
Observed: 54 kDa

Validation: N/A

Isoform: N/A

Purification: LAG3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: LAG3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: LAG3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1) , negatively regulating T-cell function and homeostasis (2) . It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3) , and acts to regulate T cell expansion (4) . LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5) .