DAPK2 Antibody

DAPK2 Antibody | 2323 | ProSci

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: DAPK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2.
This antigenic peptide is identical to the corresponding amino acids of mouse origin (1, 2) .
The immunogen is located within the last 50 amino acids of DAPK2.

Research Area: Apoptosis, Autophagy

Tested Application: E, WB, IHC-P

Application: DAPK2 antibody can be used for detection of DAPK2 by Western blot at 1 μg/mL. An approximately 42 kDa band can be detected. DAPK2 has no cross responses to DAPK1. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Specificiy: DAPK2 has no cross responses to DAPK1.

Positive Control 1: Cat. No. 1202 - A431 Cell Lysate

Positive Control 2: Cat. No. 1406 - Mouse Spleen Tissue Lysate

Positive Control 3: Cat. No. 1465 - Rat Kidney Tissue Lysate

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 42 kDa

Validation: N/A

Isoform: N/A

Purification: DAPK2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: DAPK2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: DAPK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: DAPK2 Antibody: DRP1, DRP-1, Death-associated protein kinase 2, DAP-kinase-related protein 1, DAP kinase 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: DAPK2 Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNγ induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues.