MAP1LC3A Antibody

MAP1LC3A Antibody | 7579 | ProSci

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: MAP1LC3A antibody was raised against a 10 amino acid peptide near the amino terminus of human MAP1LC3A.
The immunogen is located within the first 50 amino acids of MAP1LC3A.

Research Area: Autophagy

Tested Application: E, WB, IHC-P, IF

Application: MAP1LC3A antibody can be used for detection of MAP1LC3A by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: MAP1LC3A antibody is human specific. At least two isoforms of MAP1LC3A are known to exist. MAP1LC3A antibody is predicted to not cross-react with MAP1LC3B or MAP1LC3C.

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 3: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 14 kDa
Observed: 17 kDa

Validation: N/A

Isoform: N/A

Purification: MAP1LC3A antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: MAP1LC3A antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: MAP1LC3A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: MAP1LC3A Antibody: LC3, LC3A, ATG8E, MAP1ALC3, MAP1BLC3Autophagy-related protein LC3 A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1) . MAP1LC3A belongs to the MAP1 LC3 family of proteins that form mature complexes with MAP1A and MAP1B which are thought to be important in the formation and development of axons and dendrites (2) . MAP1LC3A is one of three isoforms of MAP1LC3, the mammalian homolog of yeast ATG8, an essential autophagy protein. These isoforms exhibit distinct expression patterns and MAP1LC3A, like MAP1LC3A but not MAP1LC3B, is post-translationally modified, suggesting the three isoforms may have different physiological functions (3) .