Bovine Plasma Protein ELISA Kit | F290

Bovine Plasma Protein ELISA Kit is intended for use in determining the presence of bovine plasma protein (BPP) pollutants in products purified from bovine source material. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The BPP assay is a two-site immunoenzymetric assay. Samples which may contain BPPs are reacted in microtiter strips coated with an affinity purified capture antibody. A second horseradish peroxidase (HRP) enzyme labeled anti-bovine plasma antibody is reacted simultaneously resulting in the formation of a sandwich complex of solid phase antibody-BPP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of total BPP present.