Bovine Transferrin ELISA Kit | F120

Bovine Transferrin ELISA Kit is intended for use in quantitating bovine transferrin. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. This assay is a two-site immunoenzymetric assay. Samples containing bovine transferrin are reacted in microtiter strips coated with an affinity purified capture antibody. A second anti-bovine transferrin antibody labeled with the enzyme horseradish peroxidase (HRP) is reacted, forming a sandwich complex of solid phase antibody-bovine transferrin-HRP labeled antibody. After a wash step to remove any unbound reactants the strips are then reacted with Tetramethylbenzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of bovine transferrin present. Accurate quantitation is achieved by comparing the signal of unknowns to bovine transferrin standards assayed at the same time.