CAP® cell HCP ELISA Kit | F820

CAP® cell HCP ELISA Kit is intended for use in determining the presence of host cell protein impurities in products manufactured by expression in the CAP® human cell line. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The CAP® cell line assay is a two-site immunoenzymetric assay. Samples containing CAP® cell HCPs are reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled anti-CAP® cell antibody (goat polyclonal) in microtiter strips coated with an affinity purified capture anti-CAP® cell antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of CAP® cell line HCPs present.