EndonucleaseGTP™ ELISA Kit | F960

EndonucleaseGTP™ ELISA Kit is intended for use in determining the presence of any endonuclease impurities in products manufactured by virus production. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The endonuclease assay is a two-site immunoenzymetric assay. Samples containing endonuclease are reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled antiendonuclease antibody (goat polyclonal) in microtiter strips coated with an affinity purified capture antiendonuclease antibody (goat polyclonal). The immunological reactions result in the formation of a sandwich complex of solid phase antibodyendonuclease-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of endonuclease enzyme present.