HeLa HCP ELISA Kit | F810

HeLa HCP ELISA Kit is intended for use in determining the presence host cell protein impurities in products manufactured by expression in HeLa host cells. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. Users should qualify this assay for use with their product samples. The HeLa Host Cell Protein assay is a two-site immunoenzymetric assay. Samples containing HeLa cell proteins are reacted in microtiter strips coated with an affinity purified capture antibody. A second HRP labeled anti-HeLa antibody is reacted simultaneously, forming a sandwich complex of solid phase antibody-HeLa HCP-800-F810, Rev. 2, 17JUL2017 HeLa HCP ELISA Product Insert 2 enzyme labeled antibody. The microtiter strips are then washed to remove any unbound reactants. After the washes, the substrate tetramethylbenzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of HeLa Host cell proteins present.