Human IgA ELISA Kit | F165

Human IgA ELISA Kit is intended for use in quantifying very low concentrations of human immunoglobulin A (hIgA). The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The hIgA assay is a two-site immunoenzymetric assay. Samples containing hIgA are reacted in microtiter strips coated with an affinity purified capture antibody. A second, horseradish peroxidase (HRP) enzyme labeled anti-hIgA antibody, is reacted simultaneously resulting in the formation of a sandwich complex of solid phase antibody-hIgA-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of hIgA present.