Human IgM ELISA Kit | F170

Human IgM ELISA Kit is intended for use in quantifying very low concentrations of human immunoglobulin M (hIgM). The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The hIgM assay is a two-site immunoenzymetric assay. Samples containing hIgM are reacted in microtiter strips coated with an affinity purified capture antibody. A second, horseradish peroxidase (HRP) enzyme labeled anti-hIgM antibody, is reacted simultaneously resulting in the formation of a sandwich complex of solid phase antibody-hIgM-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of hIgM present.