Mouse IgG1 ELISA Kit | F045

Mouse IgG1 ELISA Kit is intended for use in quantitating mouse immunoglobulin of the IgG1 isotype. The antibodies used in this kit are specific for Mouse IgG1 and will not cross react significantly with most immunoglobulins from other species or with other mouse immunoglobulins. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The Mouse IgG1 assay is a two-site immunoenzymetric assay. Samples containing mouse IgG1 are reacted in microtiter strips coated with an affinity purified IgG1 capture antibody. A second, horseradish peroxidase (HRP) enzyme labeled goat anti-mouse IgG1 antibody is reacted forming a sandwich complex of solid phase antibody- mouse immunoglobulin-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of mouse IgG1 present. Accurate quantitation is achieved by comparing the signal of unknowns to IgG1 standards assayed at the same time.