Total Goat Milk Proteins ELISA Kit | F240

Total Goat Milk Proteins ELISA Kit is intended for use in determining the presence of goat milk protein contamination in products manufactured by transgenic expression in goat milk. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. The goat milk protein assay is a two-site immunoenzymetric assay. Samples which may contain goat milk proteins are reacted in microtiter strips coated with an affinity purified capture antibody. A second horse radish peroxidase (HRP) enzyme labeled antigoat milk antibody is reacted, simultaneously resulting in the formation of a sandwich complex of solid phase antibody-milk protein-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethylbenzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of total milk proteins present.