Ultra-Sensitive Insulin ELISA Kit | F280

Ultra-Sensitive Insulin ELISA Kit is intended for use in quantitating very low concentrations of insulin, in the range of 25 to 1250pg/mL from bovine, human or porcine sources. The kit is for Research and Manufacturing Use Only and can be used to quantitate insulin in products produced by recombinant methods or in serum samples. The kit is not approved for diagnostic use in humans or animals. This insulin assay is a two-site immunoenzymetric assay. Samples containing insulin are reacted simultaneously in microtiter strips coated with an affinity purified capture antibody. A second anti-Insulin antibody labeled alkaline phosphatase is reacted forming a sandwich complex of solid phase antibody- InsulinAlkaline phosphatase labeled antibody. After a wash step to remove any unbound reactants, the strips are then reacted with p-nitrophenyl phosphate (PNPP) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of Insulin present. Accurate quantitation is achieved by comparing the signal of unknowns to Insulin standards assayed at the same time.