8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit Basic Information 

Feiyue's 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit is an ELISA reagent for detection of 8-OHdG(8-Hydroxydeoxyguanosine) , plasma or cell with sensitivity, specificity and consistency.

8-OHdG(8-Hydroxydeoxyguanosine) Introduction 

8-Hydroxyguanosine is an RNA nucleoside which is an oxidative derivative of guanosine. Measurement of the levels of 8-hydroxyguanosine is used as a biomarker of oxidative stress causing RNA damage.

8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit Test method

This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. T he concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve

Reference:

 1. Cattley RC, Glover SE (1993). "Elevated 8-hydroxydeoxyguanosine in hepatic DNA of rats following exposure to peroxisome proliferators: relationship to carcinogenesis and nuclear localization". Carcinogenesis. 14 (12): 2495–2499. doi:10.1093/carcin/14.12.2495. PMID 8269617.