FY-EH4530 Human DEFB1(Beta-defensin 1) ELISA Kit

DEFB1(Beta-defensin 1) Basic information

Beta-defensin 1 is a protein that in humans is encoded by the DEFB1 gene. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member defensin, alpha 1, and has been implicated in the pathogenesis of cystic fibrosis. Single-nucleotide polymorphisms in the DEFB1 gene were associated with plasma kynurenine concentrations in major depressive disorder patients in a genome-wide association study.

Human DEFB1(Beta-defensin 1) ELISA Kit test method

Feiyue’s Human DEFB1(Beta-defensin 1) is an ELISA reagent for detection of DEFB1(Beta-defensin 1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

This kit uses sandwich ELISA to detect the concentration of Human DEFB1(Beta-defensin 1). Human DEFB1(Beta-defensin 1)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human DEFB1(Beta-defensin 1) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human DEFB1(Beta-defensin 1) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. ENSG00000284881 GRCh38: Ensembl release 89: ENSG00000164825, ENSG00000284881 - Ensembl, May 2017

2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

3. Bensch KW, Raida M, Mägert HJ, Schulz-Knappe P, Forssmann WG (July 1995). "hBD-1: a novel beta-defensin from human plasma". FEBS Letters. 368 (2): 331–5. doi:10.1016/0014-5793(95)00687-5. PMID 7628632. S2CID 84766207.

4. "Entrez Gene: DEFB1 defensin, beta 1".