FY-EH4624 Human IL-1α(Interleukin 1 Alpha) ELISA Kit

IL-1α(Interleukin 1 Alpha) Basic information

Interleukin 1 alpha (IL-1α) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. In general, Interleukin 1 is responsible for the production of inflammation, as well as the promotion of fever and sepsis. IL-1α inhibitors are being developed to interrupt those processes and treat diseases.

IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha.

Human IL-1α(Interleukin 1 Alpha) ELISA Kit test method

Feiyue’s Human IL-1α(Interleukin 1 Alpha)  is an ELISA reagent for detection of Human IL-1α(Interleukin 1 Alpha)  in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

This kit uses sandwich ELISA to detect the concentration of Human IL-1α(Interleukin 1 Alpha). Human IL-1α(Interleukin 1 Alpha)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human IL-1α(Interleukin 1 Alpha) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human IL-1α(Interleukin 1 Alpha) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

 1.GRCh38: Ensembl release 89: ENSG00000115008 - Ensembl, May 2017

 2.GRCm38: Ensembl release 89: ENSMUSG00000027399 - Ensembl, May 2017

 3."Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

 4."Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.