FY-EH4647 Human FGF21(Fibroblast growth factor 21) ELISA Kit

FGF21(Fibroblast growth factor 21) Basic information

Fibroblast growth factor 21 is a protein that in mammals is encoded by the FGF21 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family and specifically a member of the endocrine subfamily which includes FGF23 and FGF15/19. FGF21 is the primary endogenous agonist of the FGF21 receptor, which is composed of the co-receptors FGF receptor 1 and β-Klotho. Loss of β-Klotho abolishes all effects of FGF21.

FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGFs act through a family of four FGF receptors. Binding is complicated and requires both interaction of the FGF molecule with an FGF receptor and binding to heparin through a heparin binding domain. Endocrine FGFs lack a heparin binding domain and thus can be released into the circulation.

FGF21 is a hepatokine – i.e., a hormone secreted by the liver – that regulates simple sugar intake and preferences for sweet foods via signaling through FGF21 receptors in the paraventricular nucleus of the hypothalamus and correlates with reduced dopamine neurotransmission within the nucleus accumbens.

A single-nucleotide polymorphism of the FGF21 gene – the FGF21 rs838133 variant (frequency 44.7%) – has been identified as a genetic mechanism responsible for the sweet tooth behavioral phenotype, a trait associated with cravings for sweets and high sugar consumption, in both humans and mice.

Human FGF21(Fibroblast growth factor 21) ELISA Kit test method

Feiyue’s Human FGF21(Fibroblast growth factor 21)  is an ELISA reagent for detection of FGF21(Fibroblast growth factor 21) in serum, plasma, cerebrospinal fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

This kit uses sandwich ELISA to detect the concentration of Human FGF21(Fibroblast growth factor 21). Human FGF21(Fibroblast growth factor 21)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human FGF21(Fibroblast growth factor 21) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human FGF21(Fibroblast growth factor 21) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

 1.GRCh38: Ensembl release 89: ENSG00000105550 - Ensembl, May 2017

 2.GRCm38: Ensembl release 89: ENSMUSG00000030827 - Ensembl, May 2017

 3."Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

 4."Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.