FY-EM4568 Mouse NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) ELISA Kit

NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) Basic information

Nitric oxide synthase, inducible is an enzyme which is encoded by the NOS2 gene in humans and mice.

Nitric oxide is a reactive free radical mediating in neurotransmission, antimicrobial and antitumoral activities.[citation needed] In mice, the function of Nos2 in immunity against a number of viruses, bacteria, fungi, and parasites has been well characterized, whereas in humans the role of NOS2 has remained elusive and controversial. Nos2 is important for protective immunity against CMV.Caveolin 1 has been shown to interact with Nitric oxide synthase 2A. and Rac2

Mouse NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) ELISA Kit test method

Feiyue’s Mouse NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) Elisa kit is an ELISA reagent for detection of NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

This kit uses sandwich ELISA to detect the concent Ration of Mouse NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Mouse NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concent Ration of Mouse NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1."Cloning and characterization of inducible nitric oxide synthase from mouse macrophages."

2."Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme."

3."Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line."

4."Role of NF-kappa B in the regulation of inducible nitric oxide synthase in an MTAL cell line."