FY-EM4569 Mouse IgM(Immunoglobulin M) ELISA Kit

IgM(Immunoglobulin M) Basic information

Immunoglobulin M (IgM) is one of several isotypes of antibody (also known as immunoglobulin) that are produced by vertebrates. IgM is the largest antibody, and it is the first antibody to appear in the response to initial exposure to an antigen. In the case of humans and other mammals that have been studied, the spleen, where plasmablasts responsible for antibody production reside, is the major site of specific IgM production.

Mouse IgM(Immunoglobulin M) ELISA Kit test method

This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

Reference:

1."Structure, Function, and Production of Immunoglobulin M (IgM)".

2."Immunoglobulin M an secretory immunoglobulin A: presence of common polypeptide chain different from light chains"

3."IgM molecules with and without J chain in serum and after purification, studied by ultracentrifugation, electrophoresis, and electron microsopy".

4."Addition of a mu-tailpiece to IgG results in polymeric antibodies with enhanced effector functions including complement-mediated cytolysis by IgG4"