Golimumab ELISA (SIMPONI)

This Golimumab ELISA is for determination of Golimumab in human serum, plasma and cell culture supernatant. Golimumab (CNTO 148) is a human IgG1_ monoclonal antibody, sold under the brand name Simponi. Derived from immunizing genetically engineered mice with human TNF_, Golimumab inhibits human TNF_ and effectively regulates inflammatory markers and bone metabolism. The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Golimumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Golimumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-Golimumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Golimumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.