AMH(Muellerian-inhibiting factor) Introduction

Müllerian inhibiting substance (MIS), also called anti-Müllerian hormone (AMH), is a member of the transforming growth factor-β super-family of growth and differentiation response modifiers.

Human AMH(Muellerian-inhibiting factor) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of AMH(Muellerian-inhibiting factor).AMH(Muellerian-inhibiting factor)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-AMH(Muellerian-inhibiting factor) antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of AMH(Muellerian-inhibiting factor) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

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2. Jost A. Recherches sur la differenciation sexuelle de l'embryon de lapin. Arch Anat Microsc Morphol Exp. 1947;36:271–315.

3. Jost A. Problems of fetal endocrinology: the gonadal and hypophyseal hormones. Rec Prog Horm Res. 1953;8:379–418. 

4. Price JM, Donahoe PK, Ito Y, Hendren WH., 3rd Programmed cell death in the Mullerian duct induced by Mullerian inhibiting substance. Am J Anat. 1977;149:353–375. 

5. Price JM, Donahoe PK, Ito Y. Involution of the female Mullerian duct of the fetal rat in the organ-culture assay for the detection of Mullerian Inhibiting Substance. Am J Anat. 1979;156:265–284.