CASP3(Caspase-3) Introduction

Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts.

Human CASP3(Caspase-3) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of CASP3(Caspase-3).CASP3(Caspase-3)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional CASP3(Caspase-3) antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of CASP3(Caspase-3) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference

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