Human F1+2(Prothrombin Fragment 1+2) Introduction

F 1+2 is the amino terminus fragment of the prothrombin molecule. It is a polypeptide with a half-life of approximately 90 minutes.6 F 1+2 is released from prothrombin when prothrombin is converted to thrombin by the prothrombinase complex. The prothrombinase complex consists of activated factors X and V, calcium ions, and phospholipid. F 1+2 has been utilized to assess thrombotic risk and monitor anticoagulant therapy. Increases of F 1+2 occur as a result of increased conversion of prothrombin to thrombin. F 1+2 levels are elevated in patients with deep vein thrombosis, pulmonary embolism, disseminated intravascular coagulation, septicemia, preëclampsia, eclampsia, and trauma. 

Human F1+2(Prothrombin Fragment 1+2) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Human F1+2.

Human F1+2-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human F1+2 antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich formingoptical signal measured by microplate reader.The concentration of Human F1+2 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

1. Krishnaswamy S (January 2005). "Exosite-driven substrate specificity and function in coagulation". J. Thromb. Haemost. 3 (1): 54–67. doi:10.1111/j.1538-7836.2004.01021.x. PMID 15634266. S2CID 478828.
2. Nesheim ME, Taswell JB, Mann KG (November 1979). "The contribution of bovine Factor V and Factor Va to the activity of prothrombinase". J. Biol. Chem. 254 (21): 10952–62. doi:10.1016/S0021-9258(19)86616-4. PMID 500617.
3. Di Scipio RG, Kurachi K, Davie EW (June 1978). "Activation of human factor IX (Christmas factor)". J. Clin. Invest. 61 (6): 1528–38. doi:10.1172/JCI109073. PMC 372679. PMID 659613.