Human HO1(Heme Oxygenase 1) Introduction

HO1 is a member of the heat shock protein (HSP) family identified as HSP32. HO-1 is a 32kDa enzyme which contains 288 amino acid residues encoded by the HMOX1 gene. HO-1 is not a hemoprotein as it does not contain any heme prosthetic groups. The activity of HO-1 is dependent upon NADPH-Cytochrome P450 Reductase. HO-1 is a stress-induced isoform present throughout the body with highest concentrations in the spleen, liver, and kidneys, and on the cellular level is primarily located in the endoplasmic reticulum, although it has also been reported in the mitochondria, cell nucleus, and plasma membrane. Soluble variations of HO-1 have been described. HO-1 may also serve as a chaperone protein, engage in protein-protein interactions, be secreted into the extracellular space, and participate in other cellular functions beyond its catalytic activity.

Human HO1(Heme Oxygenase 1) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Human HO1.

Human HO1-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human HO1 antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming opticalsignal measured by microplate reader.The concentration of Human HO1 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

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2. Wegiel B, Otterbein LE (2012). "Go green: the anti-inflammatory effects of biliverdin reductase". Frontiers in Pharmacology. 3: 47. doi:10.3389/fphar.2012.00047. PMC 3306015. PMID 22438844.
3. McCoubrey WK, Huang TJ, Maines MD (May 1997). "Heme oxygenase-2 is a hemoprotein and binds heme through heme regulatory motifs that are not involved in heme catalysis". The Journal of Biological Chemistry. 272 (19): 12568–12574. doi:10.1074/jbc.272.19.12568. PMID 9139709.