Human HTRA1(Serine protease HTRA1) Introduction

HTRA1 is an enzyme that in humans is encoded by the HTRA1 gene. The HTRA1 protein is composed of four distinct protein domains. They are from amino-terminus to carboxyl-terminus an Insulin-like growth factor binding domain, a kazal domain, a trypsin-like peptidase domain and a PDZ domain. This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Mutations of this gene are responsible for the development of CARASIL, a genetic form of cerebral vasculopathy.

Human HTRA1(Serine protease HTRA1) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Human HTRA1.

Human HTRA1-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human HTRA1 antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming opticalsignal measured by microplate reader.The concentration of Human HTRA1 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

1. GRCh38: Ensembl release 89: ENSG00000166033 - Ensembl, May 2017.
2. GRCm38: Ensembl release 89: ENSMUSG00000006205 - Ensembl, May 2017.
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.