Human IL-18(Interleukin 18) ELISA Kit Basic Information 

Feiyue's Human IL-18 is an ELISA reagent for detection of IL-18 antibody, plasma or cell with sensitivity, specificity and consistency.

Human IL-18(Interleukin 18) Introduction

IL-18  encoded by this gene is a proinflammatory cytokine. Many cell types, both hematopoietic cells and non-hematopoietic cells, have the potential to produce IL-18. It was first described in 1989 as a factor that induced interferon-γ (IFN-γ) production in mouse spleen cells. Originally, IL-18 production was recognized in Kupffer cells, liver-resident macrophages. However, IL-18 is constitutively expressed in non-hematopoietic cells, such as intestinal epithelial cells, keratinocytes, and endothelial cells. IL-18 can modulate both innate and adaptive immunity and its dysregulation can cause autoimmune or inflammatory diseases.

Human IL-18(Interleukin 18) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Human IL-18.

Human IL-18-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human IL-18 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human IL-18  in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000150782 - Ensembl, May 2017.
2. GRCm38: Ensembl release 89: ENSMUSG00000039217 - Ensembl, May 2017.
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.