Human IL-2(Interleukin 2) Introduction

Interleukin 2 is a kind of interleukin in cytokines, which plays an important role in the immune system. It is a protein responsible for regulating the immune activity of white blood cells (white blood cells, usually lymphocytes). The production of IL-2 is part of the body's immune response when it is infected by microorganisms to distinguish "self" from "non-self". IL-2 acts by binding to IL-2 receptors on the surface of lymphocytes.

Human IL-2(Interleukin 2) ELISA KIT Test Method

This kit uses sandwich ELISA to detect the concentration of Human IL-2.

Human IL-2-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Bovine IL-2 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich forming optical signal measured by microplate reader. The concentration of Bovine IL-2 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000109471 - Ensembl, May 2017
2. 3.0 3.1 3.2 GRCm38: Ensembl release 89: ENSMUSG00000027720 - Ensembl, May 2017
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4. Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Liao W, Lin JX, Leonard WJ. IL-2 family cytokines: new insights into the complex roles of IL-2 as a broad regulator of T helper cell differentiation. Current Opinion in Immunology. October 2011, 23 (5): 598-604. PMC 3405730. PMID 21889323. doi: 10.1016/j.coi.2011.08.003