Human IL-35(Interleukin 35) Introduciton

Secreted by regulatory T-cells (Tregs), regulatory B-cells (Bregs) or even CD8+ regulatory T cells, IL-35 suppresses inflammatory responses of immune cells. IL-35 is not constitutively expressed in tissues, but the gene encoding IL- 35 is transcribed by vascular endothelial cells, smooth muscle cells and monocytes after activation with proinflammatory stimuli. IL-35 has selective activities on different T-cell subsets; it induces proliferation of Treg cell populations but reduces activity of Th17 cell populations.

Human IL-35(Interleukin 35) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Human IL-35.

Human IL-35-specific monoclonal antibody has been pre-coated in the wells of the supplemented microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human IL-35 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich forming optical signal measured by microplate reader. The concentration of Human IL-35 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

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2. Collison LW, Workman CJ, Kuo TT, Boyd K, Wang Y, Vignali KM, et al. (November 2007). "The inhibitory cytokine IL-35 contributes to regulatory T-cell function". Nature. 450 (7169): 566 -9. Bibcode:2007Natur.450..566C. doi:10.1038/nature06306. PMID 18033300. S2CID 4425281.
3. Li X, Fang P, Yang WY, Wang H, Yang X (October 2019). "IL-35, as a newly proposed homeostasis-associated molecular pattern, plays three major functions including anti-inflammatory initiator, effector, and blocker in cardiovascular diseases". Cytokine. 122: 154076. doi:10.1016/j.cyto.2017.06.003. PMC 5741534. PMID 28648331.