Human INS(Insulin) Introduciton

Insulin is a peptide hormone produced by beta cells of the pancreatic islets; it is considered to be the main anabolic hormone of the body. It regulates the metabolism of carbohydrates, fats and protein by promoting the absorption of glucose from the blood into liver, fat and skeletal muscle cells. In these tissues the absorbed glucose is converted into either glycogen via glycogenesis or fats (triglycerides) via lipogenesis, or, in the case of the liver, into both. Glucose production and secretion by the liver is strongly inhibited by high Concentrations of insulin in the blood. Circulating insulin also affects the synthesis of proteins in a wide variety of tissues. It is therefore an anabolic hormone, promoting the conversion of small molecules in the blood into large molecules inside the cells. 

Human INS(Insulin) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Human INS.

Human INS-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human INS antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human INS in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1.GRCh38: Ensembl release 89: ENSG00000254647-Ensembl, May 2017

2.GRCm38: Ensembl release 89: ENSMUSG00000000215-Ensembl, May 2017

3."Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

4."Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

5."Insulin | Meaning of Insulin by Lexico". Lexico Dictionaries | English.