Human MCP-1(Monocyte Chemotactic Protein 1) Introduciton

Monocyte chemoattractant protein 1 (MCP1) is a small cytokine that belongs to the CC chemokine family. MCP-1 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. MCP-1 is a monomeric polypeptide, with a molecular weight of approximately 13-15 kDa depending on levels of glycosylation. MCP-1 is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. MCP-1 is primarily secreted by monocytes, macrophages and dendritic cells. Platelet derived growth factor is a major inducer of MCP-1 gene.

Human MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Human MCP-1.

Human MCP-1-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human MCP-1 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human MCP-1 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

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