Human NSE(Neuron-specific Enolase) Introduciton

Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. Gamma-enolase is a phosphopyruvate hydratase. Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Human NSE(Neuron-specific Enolase) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Human NSE.

Human NSE-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human NSE antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human NSE in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000111674 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000004267 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "ENO2 enolase 2 (gamma, neuronal)". NCBI Entrez Gene database.