Human OC/BGP(Osteocalcin) Introduciton

Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a small (49-amino-acid) noncollagenous protein hormone found in bone and dentin, first identified as a calcium-binding protein in chick bone. Because osteocalcin has gla domains, its synthesis is vitamin K dependent. In humans, osteocalcin is encoded by the BGLAP gene. Its receptors include GPRC6A, GPR158, and possibly a third, yet-to-be-identified receptor. Osteocalcin is secreted solely by osteoblasts and thought to play a role in the body's metabolic regulation. In its carboxylated form it binds calcium directly and thus concentrates in bone. In its uncarboxylated form, osteocalcin acts as a hormone in the body, signalling in the pancreas, fat, muscle, testes, and brain.

Human OC/BGP(Osteocalcin) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Human OC/BGP.

Human OC/BGP-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human OC/BGP antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human OC/BGP in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000242252 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000074489 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Hauschka, P. V.; Carr, S. A.; Biemann, K. (1982). "Primary structure of monkey osteocalcin". Biochemistry. 21 (4): 638–42. doi:10.1021/bi00533a006. PMID 6978733.