Human PCT(Procalcitonin) Introduciton

Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. It arises once preprocalcitonin is cleaved by endopeptidase. It was first identified by Leonard J. Deftos and Bernard A. Roos in the 1970s. It is composed of 116 amino acids and is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine. The level of procalcitonin in the blood stream of healthy individuals is below the limit of detection (0.01 µg/L) of clinical assays. The level of procalcitonin rises in a response to a pro-inflammatory stimulus, especially of bacterial origin. It is therefore often classed as an acute phase reactant.

Human PCT(Procalcitonin) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of Human PCT.

Human PCT-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human PCT antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human PCT in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. "Procalcitonin: Reference Range, Interpretation, Collection and Panels". 2017-03-09.
2. Deftos LJ, Roos BA, Parthemore JG (December 1975). "Calcium and skeletal metabolism". The Western Journal of Medicine. 123 (6): 447–58. PMC 1130411. PMID 1105981.
3. Dandona P, Nix D, Wilson MF, Aljada A, Love J, Assicot M, Bohuon C (December 1994). "Procalcitonin increase after endotoxin injection in normal subjects". The Journal of Clinical Endocrinology and Metabolism. 79 (6): 1605–8. doi:10.1210/jc.79.6.1605. PMID 7989463.
4. Long SS, Pickering LK, Prober CG, eds. (2012). "Bacterial infections in the neonate". Principles and Practice of Pediatric Infectious Diseases (4th ed.). Elsevier. ISBN 978-1437727029.