Human VEGF(Vascular Endothelial Growth Factor) ELISA Kit Basic Information

Feiyue's Human VEGF(Vascular Endothelial Growth Factor) ELISA Kit 
is an ELISA reagent for detection of VEGF(Vascular Endothelial Cell Growth Factor) in Human serum, plasma or cell with sensitivity, specificity and consistency.

VEGF(Vascular Endothelial Growth Factor) Introduction

Vascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF), is a signal protein produced by many cells that stimulates the formation of blood vessels. To be specific, VEGF is a sub-family of growth factors, the platelet-derived growth factor family of cystine-knot growth factors. They are important signaling proteins involved in both vasculogenesis (the de novo formation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature). It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. In mammals, the VEGF family comprises five members: VEGF-A, placenta growth factor (PGF), VEGF-B, VEGF-C and VEGF-D. The latter members were discovered after VEGF-A; before their discovery, VEGF-A was known as VEGF. A number of VEGF-related proteins encoded by viruses (VEGF-E) and in the venom of some snakes (VEGF-F) have also been discovered.

Human VEGF(Vascular Endothelial Growth Factor) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of  VEGF(Vascular Endothelial Cell Growth Factor) The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGF(Vascular Endothelial Cell Growth Factor). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VEGF(Vascular Endothelial Cell Growth Factor). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain of VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VEGF(Vascular Endothelial Cell Growth Factor) in the samples is then determined by comparing the OD of the samples to the standard curve.

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