Mouse FSH(Follicle-Stimulating Hormone) Introduction

FSH is a gonadotropin, a glycoprotein polypeptide hormone. FSH is synthesized and secreted by the gonadotropic cells of the anterior pituitary gland and regulates the development, growth, pubertal maturation, and reproductive processes of the body. FSH and luteinizing hormone (LH) work together in the reproductive system. FSH is a 35.5 kDa glycoprotein heterodimer, consisting of two polypeptide units, alpha and beta. Its structure is similar to those of luteinizing hormone (LH), thyroid-stimulating hormone (TSH), and human chorionic gonadotropin (hCG). The alpha subunits of the glycoproteins LH, FSH, TSH, and hCG are identical and consist of 96 amino acids, while the beta subunits vary. FSH has a beta subunit of 111 amino acids (FSH β), which confers its specific biologic action, and is responsible for interaction with the follicle-stimulating hormone receptor.

Mouse FSH(Follicle-Stimulating Hormone) ELISA Kit Test Method

This kit uses Competitive ELISA to detect the concentration of Mouse FSH.

The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve. 

Reference:

1.Cahoreau C, Klett D, Combarnous Y (2015-02-26). "Structure-function relationships of glycoprotein hormones and their subunits' ancestors". Frontiers in Endocrinology. 6: 26. doi:10.3389/fendo.2015.00026. PMC 4341566. PMID 25767463.

2."Follicle-Stimulating Hormone". WebMD.

3.Bowen R. "Luteinizing and Follicle Stimulating Hormones". www.vivo.colostate.edu. Retrieved 2019-05-06.