Mouse IL-12(Interleukin 12) Introduction

IL-12 is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils. IL-12 belongs to the family of interleukin-12. IL-12 family is unique in comprising the only heterodimeric cytokines, which includes IL-12, IL-23, IL-27 and IL-35]. Despite sharing many structural features and molecular partners, they mediate surprisingly diverse functional effects. IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.

Mouse IL-12(Interleukin 12) ELISA Kit Test Method

This kit uses sandwich ELISA to detect the concentration of Mouse IL-12.

Mouse IL-12-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Mouse IL-12 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Mouse IL-12 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

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