ADP/Acrp30(Adiponectin) Introduction 

Adiponectin (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is a protein hormone and adipokine, which is involved in regulating glucose levels as well as fatty acid breakdown. In humans it is encoded by the ADIPOQ gene and it is produced in primarily in adipose tissue, but also in muscle, and even in the brain.

Rat ADP/Acrp30(Adiponectin) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of ADP/Acrp30(Adiponectin).ADP/Acrp30(Adiponectin)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-ADP/Acrp30(Adiponectin) antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of ADP/Acrp30(Adiponectin) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

Reference:

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